An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts----中国科学院藻类生物学重点实验室

论文名称	An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts
作者	Mevaere, Jimmy; Goulard, Christophe; Schneider, Olha; Sekurova, Olga N.; Ma, Haiyan; Zirah, Severine; Afonso, Carlos; Rebuffat, Sylvie; Zotchev, Sergey B.; Li, Yanyan
摘要	Lasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria. They are characterized by an unusual lariat-knot structure. Targeted genome scanning revealed a wide diversity of lasso peptides encoded in actinobacterial genomes, but cloning and heterologous expression of these clusters turned out to be problematic. To circumvent this, we developed an orthogonal expression system for heterologous production of actinobacterial lasso peptides in Streptomyces hosts based on a newly-identified regulatory circuit from Actinoalloteichus fjordicus. Six lasso peptide gene clusters, mainly originating from marine Actinobacteria, were chosen for proof-of-concept studies. By varying the Streptomyces expression hosts and a small set of culture conditions, three new lasso peptides were successfully produced and characterized by tandem MS. The newly developed expression system thus sets the stage to uncover and bioengineer the chemodiversity of actinobacterial lasso peptides. Moreover, our data provide some considerations for future bioprospecting efforts for such peptides.
卷	8
期
页码
发表时间	2018
刊物名称	SCIENTIFIC REPORTS
全文链接	http://dx.doi.org/10.1038/s41598-018-26620-0